Molecular Identification of Coxiella burnetii and Study Physical Features in Raw Milk of Goat in Karbala Province

This study aims to detect Coxiella burnetii in Karbala Province using Chemical Examination of milk and confirmation of infection by polymerase chain reaction test (PCR). The prevalence of Coxiella burnetii was found to be 10% targeting 16S rRNA genes from five positive samples, according to the findings of the fifty raw milk samples that were obtained from fifty goats from various regions within the main districts of Karbala city between September 2023 and March 2024. It has been demonstrated by the findings of this study that there is a large amount of C. burnetii DNA present in goat raw milk supplies. The finding of the PH was 8, while the results of the catalase were recorded as positive results. On the other hand, the results of the chloride exhibited a milky-gray color. In conclusion, our findings confirmed the prevalence of C. burnetii among Iraqi goat, suggesting that these animals might be a source of the pathogen for humans and other animal species.