New 5-substituted-1,3,4-thiadiazole-2(3H)-thione Derivatives: Synthesis and Their In vitro Antimicrobial Properties

In this paper a new series of 1,3,4-thiadiazole derivatives have been designed, synthesized and evaluated as antimicrobial agents. New compounds were prepared by the reaction of 5-amino-1,3,4-thiadiazole-2(3H)-thione with appropriate aldehydes. The structures of the obtained compounds were confirmed by means of 1H NMR and 13C NMR. All synthesized compounds were tested for their in vitro antibacterial and antifungal activities using the broth microdilution method. Our results showed that the three of synthesized compounds had bactericidal or fungicidal effect against reference strains of Gram-positive bacteria, mainly opportunistic S. epidermidis, M. luteus, Bacillus spp., and yeast belonging to Candida spp.